mitochondrial fission 1 protein (Proteintech)
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mitochondrial fission 1 protein
Mitochondrial Fission 1 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitochondrial+fission+1/FIS1+Polyclonal+antibody/pm41594656-89-27-33
Average 96 stars, based on 345 article reviews
Mitochondrial Fission 1 Protein, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 345 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mitochondrial+fission+1/FIS1+Polyclonal+antibody/pm41594656-89-27-33
Average 96 stars, based on 345 article reviews
mitochondrial fission 1 protein - by Bioz Stars,
2026-10
96/100 stars
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Saline:Article Title: Mitochondrial Dysfunction and Morphological Abnormality in Keloid Fibroblasts Article Snippet: Protein concentration of lysates was determined by a BCA Protein Assay Kit (CWbiotech, Beijing, China), and Western blotting was performed as previously described.10 Briefly, proteins were separated by 10% sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred onto a 0.45 lm polyvinylidene fluoride membrane (Millipore, Billerica, MA). .. Membranes were blocked in Tris-buffered saline containing 0.002 g/L Tween 20 (TBST) with 5% bovine serum albumin for 1 h and incubated overnight at 4 C in TBST with primary antibodies against citrate synthase (CS), isocitrate dehydrogenase 2 (IDH2), succinate dehydrogenase complex flavoprotein subunit A (SDHA) (1:1,000; Abcam, Cambridge, UK), Incubation:Article Title: Mitochondrial Dysfunction and Morphological Abnormality in Keloid Fibroblasts Article Snippet: Protein concentration of lysates was determined by a BCA Protein Assay Kit (CWbiotech, Beijing, China), and Western blotting was performed as previously described.10 Briefly, proteins were separated by 10% sodium dodecyl sulfate– polyacrylamide gel electrophoresis and transferred onto a 0.45 lm polyvinylidene fluoride membrane (Millipore, Billerica, MA). .. Membranes were blocked in Tris-buffered saline containing 0.002 g/L Tween 20 (TBST) with 5% bovine serum albumin for 1 h and incubated overnight at 4 C in TBST with primary antibodies against citrate synthase (CS), isocitrate dehydrogenase 2 (IDH2), succinate dehydrogenase complex flavoprotein subunit A (SDHA) (1:1,000; Abcam, Cambridge, UK), Article Title: Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4‑mediated ferritinophagy. Article Snippet: A total of 25 μg total protein was added and subsequently subjected to separation by SdS‐PAGE on 10% gels, followed by the transfer of the separated proteins onto polyvinylidene difluoride membranes (MilliporeSigma). .. After blocking with 5% BSA (Wuhan Boster Biological Technology, Ltd.) for 30 min at room temperature, the membranes were incubated with primary antibodies against Nrf2 (cat. no. 16396‐1‐AP; 1:1,000; Proteintech Group, Inc.), heme oxygenase (HO)‐1 (cat. no. BM4010; 1:1,000; Wuhan Boster Biological Technology, Ltd.), nuclear receptor coactivator 4 (NcOA4; cat. no. ab86707; 1:1,000; Abcam), Lc3B (cat. no. 4108; 1:1,000; cell Signaling Technology, Inc.), dynamin‐related protein 1 (drp1; cat. no. 12957‐1‐AP; 1:1,000; Proteintech Group, Inc.), mitochondrial fission factor (MFF; 1:1,000; cat. no. 84580; cell Signaling Technology, Inc.), ferritin heavy chain 1 (FTH1; cat. no. ab75973; 1:1,000; Abcam), type II collagen (cOL2; cat. no. 28459‐1‐AP; 1:1,000; Proteintech Group, Inc.), SOX9 (cat. no. A00177‐2; 1:500; Wuhan Boster Biological Technology, Ltd), Parkin (cat. no. 14060‐1‐AP; 1:1,000; Proteintech Group, Inc.), matrix metalloproteinase (MMP)3 (cat. no. 17873‐1‐AP; 1:1,000; Proteintech Group, Inc.), MMP13 (cat. no. 18165‐1‐AP; 1:1,000; Proteintech Group, Inc.), solute carrier family 7 member 11 (SLc7A11; cat. no. 26864‐1‐AP; 1:1,000; Proteintech Group, Inc.), cOL10 (cat. no. BA 2023; 1:500; Wuhan Boster Biological Technology, Ltd.), Article Title: Mitochondrial dysfunction in mesenchymal stem cells impairs osteogenesis in radiation-induced bone injury via Ca 2+ -NFATc1-Fis1 pathway. Article Snippet: .. Briefly, the slides were incubated with antibodies for osteocalcin (OCN, 1:100, Santa Cruz, USA), tartrateresistant acid phosphatase (TRAP, 1:100, Servicebio, China) and peroxisome proliferator-activated receptor-γ (PPAR-γ, 1:100, Santa Cruz, USA) and Article Title: Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4-mediated ferritinophagy Article Snippet: A total of 25 μ g total protein was added and subsequently subjected to separation by SDS-PAGE on 10% gels, followed by the transfer of the separated proteins onto polyvinylidene difluoride membranes (MilliporeSigma). .. After blocking with 5% BSA (Wuhan Boster Biological Technology, Ltd.) for 30 min at room temperature, the membranes were incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:1,000; Proteintech Group, Inc.), heme oxygenase (HO)-1 (cat. no. BM4010; 1:1,000; Wuhan Boster Biological Technology, Ltd.), nuclear receptor coactivator 4 (NCOA4; cat. no. ab86707; 1:1,000; Abcam), LC3B (cat. no. 4108; 1:1,000; Cell Signaling Technology, Inc.), dynamin-related protein 1 (Drp1; cat. no. 12957-1-AP; 1:1,000; Proteintech Group, Inc.), mitochondrial fission factor (MFF; 1:1,000; cat. no. 84580; Cell Signaling Technology, Inc.), ferritin heavy chain 1 (FTH1; cat. no. ab75973; 1:1,000; Abcam), type II collagen (COL2; cat. no. 28459-1-AP; 1:1,000; Proteintech Group, Inc.), SOX9 (cat. no. A00177-2; 1:500; Wuhan Boster Biological Technology, Ltd), Parkin (cat. no. 14060-1-AP; 1:1,000; Proteintech Group, Inc.), matrix metalloproteinase (MMP)3 (cat. no. 17873-1-AP; 1:1,000; Proteintech Group, Inc.), MMP13 (cat. no. 18165-1-AP; 1:1,000; Proteintech Group, Inc.), solute carrier family 7 member 11 (SLC7A11; cat. no. 26864-1-AP; 1:1,000; Proteintech Group, Inc.), COL10 (cat. no. BA 2023; 1:500; Wuhan Boster Biological Technology, Ltd.), Article Title: Mitochondrial dysfunction in mesenchymal stem cells impairs osteogenesis in radiation-induced bone injury via Ca 2+ -NFATc1-Fis1 pathway Article Snippet: .. Briefly, the slides were incubated with antibodies for osteocalcin (OCN, 1:100, Santa Cruz, USA), tartrate-resistant acid phosphatase (TRAP, 1:100, Servicebio, China) and peroxisome proliferator-activated receptor-γ (PPAR-γ, 1:100, Santa Cruz, USA) and Blocking Assay:Article Title: Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4‑mediated ferritinophagy. Article Snippet: A total of 25 μg total protein was added and subsequently subjected to separation by SdS‐PAGE on 10% gels, followed by the transfer of the separated proteins onto polyvinylidene difluoride membranes (MilliporeSigma). .. After blocking with 5% BSA (Wuhan Boster Biological Technology, Ltd.) for 30 min at room temperature, the membranes were incubated with primary antibodies against Nrf2 (cat. no. 16396‐1‐AP; 1:1,000; Proteintech Group, Inc.), heme oxygenase (HO)‐1 (cat. no. BM4010; 1:1,000; Wuhan Boster Biological Technology, Ltd.), nuclear receptor coactivator 4 (NcOA4; cat. no. ab86707; 1:1,000; Abcam), Lc3B (cat. no. 4108; 1:1,000; cell Signaling Technology, Inc.), dynamin‐related protein 1 (drp1; cat. no. 12957‐1‐AP; 1:1,000; Proteintech Group, Inc.), mitochondrial fission factor (MFF; 1:1,000; cat. no. 84580; cell Signaling Technology, Inc.), ferritin heavy chain 1 (FTH1; cat. no. ab75973; 1:1,000; Abcam), type II collagen (cOL2; cat. no. 28459‐1‐AP; 1:1,000; Proteintech Group, Inc.), SOX9 (cat. no. A00177‐2; 1:500; Wuhan Boster Biological Technology, Ltd), Parkin (cat. no. 14060‐1‐AP; 1:1,000; Proteintech Group, Inc.), matrix metalloproteinase (MMP)3 (cat. no. 17873‐1‐AP; 1:1,000; Proteintech Group, Inc.), MMP13 (cat. no. 18165‐1‐AP; 1:1,000; Proteintech Group, Inc.), solute carrier family 7 member 11 (SLc7A11; cat. no. 26864‐1‐AP; 1:1,000; Proteintech Group, Inc.), cOL10 (cat. no. BA 2023; 1:500; Wuhan Boster Biological Technology, Ltd.), Article Title: Nrf2 protects against cartilage endplate degeneration through inhibiting NCOA4-mediated ferritinophagy Article Snippet: A total of 25 μ g total protein was added and subsequently subjected to separation by SDS-PAGE on 10% gels, followed by the transfer of the separated proteins onto polyvinylidene difluoride membranes (MilliporeSigma). .. After blocking with 5% BSA (Wuhan Boster Biological Technology, Ltd.) for 30 min at room temperature, the membranes were incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:1,000; Proteintech Group, Inc.), heme oxygenase (HO)-1 (cat. no. BM4010; 1:1,000; Wuhan Boster Biological Technology, Ltd.), nuclear receptor coactivator 4 (NCOA4; cat. no. ab86707; 1:1,000; Abcam), LC3B (cat. no. 4108; 1:1,000; Cell Signaling Technology, Inc.), dynamin-related protein 1 (Drp1; cat. no. 12957-1-AP; 1:1,000; Proteintech Group, Inc.), mitochondrial fission factor (MFF; 1:1,000; cat. no. 84580; Cell Signaling Technology, Inc.), ferritin heavy chain 1 (FTH1; cat. no. ab75973; 1:1,000; Abcam), type II collagen (COL2; cat. no. 28459-1-AP; 1:1,000; Proteintech Group, Inc.), SOX9 (cat. no. A00177-2; 1:500; Wuhan Boster Biological Technology, Ltd), Parkin (cat. no. 14060-1-AP; 1:1,000; Proteintech Group, Inc.), matrix metalloproteinase (MMP)3 (cat. no. 17873-1-AP; 1:1,000; Proteintech Group, Inc.), MMP13 (cat. no. 18165-1-AP; 1:1,000; Proteintech Group, Inc.), solute carrier family 7 member 11 (SLC7A11; cat. no. 26864-1-AP; 1:1,000; Proteintech Group, Inc.), COL10 (cat. no. BA 2023; 1:500; Wuhan Boster Biological Technology, Ltd.), |
